Production of Insect Toxin Beauvericin from Entomopathogenic Fungi Cordyceps militaris by Heterologous Expression of Global Regulator
Cordyceps militaris is one of entomopathogenic fungi species that is well known to be a traditional medicine in China for decades. Although the pharmaceutical and/or toxic properties of C. militaris has attracted attention as a promising resource for finding bioactive compounds, only a few substance...
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| Main Authors: | , , |
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| Format: | EJournal Article |
| Published: |
Faculty of Agriculture University of Brawijaya in collaboration with PERAGI,
2017-12-20.
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| Online Access: | Get fulltext |
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| LEADER | 02476 am a22003133u 4500 | ||
|---|---|---|---|
| 001 | AGRIVITA__1727_907 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Rachmawati, Rina |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Kinoshita, Hiroshi |e author |
| 700 | 1 | 0 | |a Nihira, Takuya |e author |
| 245 | 0 | 0 | |a Production of Insect Toxin Beauvericin from Entomopathogenic Fungi Cordyceps militaris by Heterologous Expression of Global Regulator |
| 260 | |b Faculty of Agriculture University of Brawijaya in collaboration with PERAGI, |c 2017-12-20. | ||
| 500 | |a https://agrivita.ub.ac.id/index.php/agrivita/article/view/1727 | ||
| 520 | |a Cordyceps militaris is one of entomopathogenic fungi species that is well known to be a traditional medicine in China for decades. Although the pharmaceutical and/or toxic properties of C. militaris has attracted attention as a promising resource for finding bioactive compounds, only a few substances including cordycepin have been reported so far. In the previous report heterologous expression of LaeA, a global regulator for secondary metabolites production in fungi, has been succeeded in C. militaris. The LaeA-engineered transformants are proved to produce new and/or elevated production of secondary metabolites, as detected by HPLC analysis. In order to further characterize the secondary metabolites that were being significantly produced by LaeA transformant, HPLC profiling and structure elucidation by proton NMR were conducted in two target compounds, designated as compound 1 and compound 2. Compound 1 possessed the highly similar characters to insect toxin beauvericin in UV spectrum, molecular weight, and retention time in HPLC analysis. Proton NMR analysis revealed that compound 1 had the same proton signals as beauvericin. | ||
| 540 | |a Copyright (c) 2017 Universitas Brawijaya | ||
| 540 | |a http://creativecommons.org/licenses/by-nc/4.0 | ||
| 546 | |a eng | ||
| 690 | |||
| 690 | |a Genetic engineering; LaeA; Transformation system | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n AGRIVITA, Journal of Agricultural Science; Vol 40, No 1 (2018): FEBRUARY; 177-184 | |
| 786 | 0 | |n 2477-8516 | |
| 786 | 0 | |n 0126-0537 | |
| 786 | 0 | |n 10.17503/agrivita.v40i1 | |
| 787 | 0 | |n https://agrivita.ub.ac.id/index.php/agrivita/article/view/1727/907 | |
| 856 | 4 | 1 | |u https://agrivita.ub.ac.id/index.php/agrivita/article/view/1727/907 |z Get fulltext |