Prevalence of Puroindoline Genes and Their Impact on Quality Traits in A Diverse Germplasm of Wheat Genotypes

Grain hardness is an imperative attribute that determines the end-use quality of wheat. Variation in grain hardness is usually controlled by Puroindoline (pin-a and pin-b) genes located on the 5D chromosome. The study was aimed to reveal different mutations in Puroindoline genes utilizing the STS-ma...

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Main Authors: Khurshid, Mariam (Author), Ahmad, Munir (Author)
Other Authors: PMAS-Arid Agriculture University, Rawalpindi, pakistan (Contributor), higher Education COMM (Contributor)
Format: EJournal Article
Published: Faculty of Agriculture University of Brawijaya in collaboration with PERAGI, 2021-08-20.
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LEADER 02769 am a22003133u 4500
001 AGRIVITA__3018_1410
042 |a dc 
100 1 0 |a Khurshid, Mariam  |e author 
100 1 0 |a PMAS-Arid Agriculture University, Rawalpindi, pakistan  |e contributor 
100 1 0 |a higher Education COMM  |e contributor 
700 1 0 |a Ahmad, Munir  |e author 
245 0 0 |a Prevalence of Puroindoline Genes and Their Impact on Quality Traits in A Diverse Germplasm of Wheat Genotypes 
260 |b Faculty of Agriculture University of Brawijaya in collaboration with PERAGI,   |c 2021-08-20. 
500 |a https://agrivita.ub.ac.id/index.php/agrivita/article/view/3018 
520 |a Grain hardness is an imperative attribute that determines the end-use quality of wheat. Variation in grain hardness is usually controlled by Puroindoline (pin-a and pin-b) genes located on the 5D chromosome. The study was aimed to reveal different mutations in Puroindoline genes utilizing the STS-marker approach and their association with important quality attributes in 100 hexaploid wheat genotypes (96 from Pakistan and 4 from CIMMYT). Overall, seven puroidoline genes were identified. Among them Pina-d1b(null) (85%) was most common while Pinb-d1i (1%) and Pinb-d1ab (1%) were most rare gene. Out of 100 genotypes, 97 had hard texture either with single or double mutant pin-genes, while three had a soft texture with wild type (Pinad1a/Pinb-d1a) pin-genes. All four quality attributes revealed a vast deviation among germplasm, while their correlation analysis revealed the highest association (r=0.71) between thousand-grain weight and protein content. In addition, three out of four quality traits, i.e. thousandgrain weight, SDS-sedimentation value and protein content, showed the highest mean values for double mutant (Pina-d1b/Pinb-d1b) followed by single mutant, i.e. Pina-d1b. The present study facilitates breeders for varietal selection (hard or soft) according to end-use quality and offers valuable information for improving wheat quality. 
540 |a Copyright (c) 2021 The Author(s) 
540 |a http://creativecommons.org/licenses/by-nc/4.0 
546 |a eng 
690 |a plant breeding and genetics 
690 |a End-use quality; Grain hardness; SDS-sedimentation value; STS-marker approach 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n AGRIVITA, Journal of Agricultural Science; Vol 43, No 3 (2021); 454-465 
786 0 |n 2477-8516 
786 0 |n 0126-0537 
786 0 |n 10.17503/agrivita.v43i3 
787 0 |n https://agrivita.ub.ac.id/index.php/agrivita/article/view/3018/1410 
856 4 1 |u https://agrivita.ub.ac.id/index.php/agrivita/article/view/3018/1410  |z Get fulltext