Polymorphisms of TLR4 Asp299Gly and TNF-α -308G/A in Leptospirosis
Background : TLR4 Asp299Gly and TNF-α -308G/A polymorphisms have been shown to be associated with increased susceptibility and severity of infection. TLR4 Asp299Gly polymorphism could affect the host's ability to respond to leptospira sp. TNF-α -308G/A polymorphism, is associated with the high...
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| Format: | EJournal Article |
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Faculty of Medicine, Universitas Diponegoro,
2016-07-30.
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| LEADER | 02639 am a22002773u 4500 | ||
|---|---|---|---|
| 001 | BTR_UNDIP_580_1013 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Farhanah, Nur |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Gasem, Muhammad Hussein |e author |
| 700 | 1 | 0 | |a Faradz, Sultana MH |e author |
| 245 | 0 | 0 | |a Polymorphisms of TLR4 Asp299Gly and TNF-α -308G/A in Leptospirosis |
| 260 | |b Faculty of Medicine, Universitas Diponegoro, |c 2016-07-30. | ||
| 500 | |a https://ejournal2.undip.ac.id/index.php/jbtr/article/view/580 | ||
| 520 | |a Background : TLR4 Asp299Gly and TNF-α -308G/A polymorphisms have been shown to be associated with increased susceptibility and severity of infection. TLR4 Asp299Gly polymorphism could affect the host's ability to respond to leptospira sp. TNF-α -308G/A polymorphism, is associated with the high producer of TNF-α.Methods : Total of 36 leptospirosis patients (IgM anti leptospira and MAT positive) and healthy individual with the equal number were included. The polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using site spesific restriction enzyme.Results : Distribution of homozygous wild-type TLR4 Asp299Gly polymorphism was higher in both of groups ( 94.5:97.2%.) and homozygous mutant allele was absent. There was not significantly difference of TLR4 Asp299Gly in leptospirosis patients and healthy group ( ρ=1.00; OR 0.5; 95%CI, 0.04-5.6) and between mild and severe leptospirosis (ρ=0.54; OR 1.54 ; 95% CI, 1.20-1.98). The presence of homozygous wild-type TNF-α -308G/A polymorphism was higher between leptospirosis patients and healthy group (100:94.5%) andhomozygous mutant allele was not found in both of the groups. No significantly different of TNF-α -308G>A polymorphism between leptospirosis patient and healthy group (ρ=0.49).Conclusions : In this study, the polymorphisms of TLR4 Asp299Gly and TNF-α -308G/A were not associated with the susceptibility and severity of leptospirosis. | ||
| 540 | |a Copyright (c) 2016 Journal of Biomedicine and Translational Research | ||
| 546 | |a eng | ||
| 690 | |a Leptospirosis; TLR4 Asp299 Gly polymorphis; TNF-α -308G/A polymorphism | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |2 local | |
| 786 | 0 | |n Journal of Biomedicine and Translational Research; Vol 2, No 1 (2016): July 2016; 17-22 | |
| 786 | 0 | |n 2503-2178 | |
| 786 | 0 | |n 10.14710/jbtr.v2i1 | |
| 787 | 0 | |n https://ejournal2.undip.ac.id/index.php/jbtr/article/view/580/1013 | |
| 856 | 4 | 1 | |u https://ejournal2.undip.ac.id/index.php/jbtr/article/view/580/1013 |z Get Fulltext |