THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN

ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and bel...

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Main Authors: Takdir, Moh (Author), ismaya, Ismaya (Author), Bintara, Sigit (Author)
Format: EJournal Article
Published: Faculty of Animal Science, Universitas Gadjah Mada, 2017-02-27.
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001 BuletinPeternakan_UGM_9130_pdf
042 |a dc 
100 1 0 |a Takdir, Moh  |e author 
100 1 0 |e contributor 
700 1 0 |a ismaya, Ismaya  |e author 
700 1 0 |a Bintara, Sigit  |e author 
245 0 0 |a THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN 
260 |b Faculty of Animal Science, Universitas Gadjah Mada,   |c 2017-02-27. 
500 |a https://jurnal.ugm.ac.id/buletinpeternakan/article/view/9130 
520 |a ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and below each medium fraction treatment. There are treatment egg white albumin as separation medium, each medium consisting of fractions top and bottom fraction with different concentration: 1) P0 = sperma before separation (control); 2) P1 = 10% above fraction + 30% lower fraction; P2 = 25% + 45%; P3 = 25% + 75%. Data proportion of X and Y, viability and motility were analyzed statistically by Completely Randomized Design patern in the direction followed by Duncan's Multiple Range Test for data with a real difference. Separation with egg white albumin affect significantly increased the proportion of spermatozoa X and Y (P≤0.05), but tends to decrease the viability and motility of spermatozoa.The proportion of spermatozoa X and Y was highest in treatment P3,76.76% of spermatozoa X (fraction above 25%) and 79.81% spermatozoa Y (75% lower fraction), with an average viability obtained respectively 68,9% (fraction above) and 59,7% (bottom fraction), motility 77,5% (fraction above) dan 84,0% (bottom fraction). It was concluded that the egg white albumin is very effective in changing the proportions of X and Y ram sperm with the quality of spermatozoa after separation feasible for applications insemination or processed into frozen semen.  (Keywords: Garut ram, White egg albumin, Spermatozoa X and Y)  
540 |a Copyright (c) 2017 Buletin Peternakan 
540 |a http://journal.ugm.ac.id/buletinpeternakan/pages/view/Publication%20Ethics%20and%20Malpractice%20Statement 
546 |a eng 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Buletin Peternakan; Vol 41, No 1 (2017): BULETIN PETERNAKAN VOL. 41 (1) FEBRUARI 2017; 1-7 
786 0 |n 2407-876X 
786 0 |n 0126-4400 
786 0 |n 10.21059/buletinpeternak.v41i1 
787 0 |n https://jurnal.ugm.ac.id/buletinpeternakan/article/view/9130/pdf 
856 4 1 |u https://jurnal.ugm.ac.id/buletinpeternakan/article/view/9130/pdf  |z Get Online