Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells

The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore iden...

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Main Authors: Mariya, Silmi (Author), Dewi, Fitriya Nur Annisa (Author), Suparto, Irma Herawati (Author), Wilkerson, Gregory K. (Author), Cline, J. Mark (Author), Permanawati, (Author), Iskandriati, Diah (Author), Budiarsa, I Nengah (Author), Sajuthi, Dondin (Author)
Format: EJournal Article
Published: Bogor Agricultural University, Indonesia, 2018-03-26.
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LEADER 02708 am a22003613u 4500
001 HAYATI_20808_14254
042 |a dc 
100 1 0 |a Mariya, Silmi  |e author 
700 1 0 |a Dewi, Fitriya Nur Annisa  |e author 
700 1 0 |a Suparto, Irma Herawati  |e author 
700 1 0 |a Wilkerson, Gregory K.  |e author 
700 1 0 |a Cline, J. Mark  |e author 
700 1 0 |a Permanawati, .  |e author 
700 1 0 |a Iskandriati, Diah  |e author 
700 1 0 |a Budiarsa, I Nengah  |e author 
700 1 0 |a Sajuthi, Dondin  |e author 
245 0 0 |a Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells 
260 |b Bogor Agricultural University, Indonesia,   |c 2018-03-26. 
500 |a https://journal.ipb.ac.id/index.php/hayati/article/view/20808 
520 |a The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore identified the expression of markers for stemness and estrogenreceptor-associated activities. We found that the primary culture can be successfully subcultured to at least 3 passages, primarily epithelial-like in morphology, the cultured cells remained heterogenous in phenotype as they expressed epithelial cell markers CD24, CK18, and marker for fibroblast S1004A. Importantly, the cell population also consistently expressed the markers of mammary stem cells (ITGB1 or CD29 and ITGA6 or CD49f), mesenchymal stem cells (CD73 and CD105) and pluripotency (NANOG, OCT4, SOX2). In addition to this, the cells were also positive for Estrogen Receptor (ER), and ER-activated marker Trefoil Factor 1, suggesting an estrogen responsiveness of the culture model. These results indicate that our cell culture model is a reliable model for acquiring a population of cells with mammary stem cell properties and that these cultures may also serve as a reservoir from which more purified populations of stem cell populations can be isolated in the future. 
546 |a eng 
690 |a breast cancer 
690 |a mammary gland 
690 |a nonhuman primate 
690 |a stem cells 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n HAYATI Journal of Biosciences; Vol. 24 No. 3 (2017): July 2017; 136 
786 0 |n 2086-4094 
786 0 |n 1978-3019 
787 0 |n https://journal.ipb.ac.id/index.php/hayati/article/view/20808/14254 
856 4 1 |u https://journal.ipb.ac.id/index.php/hayati/article/view/20808/14254  |z Get fulltext