Expression Analysis of 1-aminocyclopropane-1-carboxylic Acid Oxidase Genes in Chitosan-Coated Banana

Banana is a climacteric fruit in which ethylene plays an important role in the regulation of the ripening process. Though it is the most produced fruit in Indonesia, the current post-harvest technologies for exporting this fruit are not economically friendly. Chitosan is one of economical biopolymer...

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Main Authors: Yamamoto, Kana (Author), Amalia, Annisa (Author), Putri, Sastia P. (Author), Fukusaki, Eiichiro (Author), Dwivany, Fenny M. (Author)
Format: EJournal Article
Published: Bogor Agricultural University, Indonesia, 2018-10-04.
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LEADER 02468 am a22003373u 4500
001 HAYATI_23484_15289
042 |a dc 
100 1 0 |a Yamamoto, Kana  |e author 
700 1 0 |a Amalia, Annisa  |e author 
700 1 0 |a Putri, Sastia P.  |e author 
700 1 0 |a Fukusaki, Eiichiro  |e author 
700 1 0 |a Dwivany, Fenny M.  |e author 
245 0 0 |a Expression Analysis of 1-aminocyclopropane-1-carboxylic Acid Oxidase Genes in Chitosan-Coated Banana 
260 |b Bogor Agricultural University, Indonesia,   |c 2018-10-04. 
500 |a https://journal.ipb.ac.id/index.php/hayati/article/view/23484 
520 |a Banana is a climacteric fruit in which ethylene plays an important role in the regulation of the ripening process. Though it is the most produced fruit in Indonesia, the current post-harvest technologies for exporting this fruit are not economically friendly. Chitosan is one of economical biopolymer for edible coating which can extend fruit shelf-life. However, little study focused on the effect of chitosan coating has been done on gene expression level. In this study, the expression levels of several 1-aminocyclopropan-1-carboxylic acid oxidase (ACO) genes, which is an enzyme to convert 1-aminocyclopropan-1-carboxylic acid to ethylene in banana were analyzed on day 0, 1, 3, 5, 7, and 9 after ethylene treatment. As a result, one gene (ID: Ma01_t11540.1) had a similar expression pattern in both control and chitosan-coated bananas while the other genes (ID: Ma03_t02700.1, Ma05_t09360.1, Ma06_t02600.1, Ma10_t01130.1) showed different expression patterns. Among these genes, two genes (ID: Ma05_t09360.1, Ma10_t01130.1) were expressed higher than the other genes and the peak was observed on day 3. It was indicated that chitosan coating might activate the ethylene biosynthesis pathway in banana while it delayed fruit ripening. 
546 |a eng 
690 |a ACO 
690 |a ethylene 
690 |a chitosan 
690 |a edible coating 
690 |a banana ripening 
690 |a real-time PCR 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n HAYATI Journal of Biosciences; Vol. 25 No. 1 (2018): January 2018; 18 
786 0 |n 2086-4094 
786 0 |n 1978-3019 
787 0 |n https://journal.ipb.ac.id/index.php/hayati/article/view/23484/15289 
856 4 1 |u https://journal.ipb.ac.id/index.php/hayati/article/view/23484/15289  |z Get fulltext