The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of comm...

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Main Authors: Widyastuti, Anna (Author), Putrika, Afiatry (Author), Dwiranti, Astari (Author), Salamah, Andi (Author), Hemelda, Niarsi Merry (Author), Handayani, Windri (Author)
Format: EJournal Article
Published: Bogor Agricultural University, Indonesia, 2021-04-01.
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LEADER 02329 am a22002773u 4500
001 HAYATI_36779_22010
042 |a dc 
100 1 0 |a Widyastuti, Anna   |e author 
700 1 0 |a Putrika, Afiatry   |e author 
700 1 0 |a Dwiranti, Astari   |e author 
700 1 0 |a Salamah, Andi   |e author 
700 1 0 |a Hemelda, Niarsi Merry   |e author 
700 1 0 |a Handayani, Windri   |e author 
245 0 0 |a The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp. 
260 |b Bogor Agricultural University, Indonesia,   |c 2021-04-01. 
500 |a https://journal.ipb.ac.id/index.php/hayati/article/view/36779 
520 |a In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp. 
546 |a eng 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n HAYATI Journal of Biosciences; Vol. 28 No. 2 (2021): April 2021; 110 
786 0 |n 2086-4094 
786 0 |n 1978-3019 
787 0 |n https://journal.ipb.ac.id/index.php/hayati/article/view/36779/22010 
856 4 1 |u https://journal.ipb.ac.id/index.php/hayati/article/view/36779/22010  |z Get fulltext