The Identification of Carotenoids and Testing of Carotenoid Antioxidants from Sand Lobster (Panulirus homarus) Egg Extract

Sand lobsters (Panulirus homarus) are organisms that play an important role in supporting a society's economy. Lobsters have a business appeal due to their high demand, enjoyable taste, as well as significant nutritional content. It is interesting that sand lobster eggs have a yellow orange col...

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Main Authors: Ngginak, James (Author), Mangibulude, Jubhar C (Author), Rondonuwu, Ferdy S (Author)
Format: EJournal Article
Published: Marine Science Department Diponegoro University, 2017-08-22.
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LEADER 02832 am a22002773u 4500
001 IJMS_UNDIP_15685_pdf
042 |a dc 
100 1 0 |a Ngginak, James  |e author 
100 1 0 |e contributor 
700 1 0 |a Mangibulude, Jubhar C  |e author 
700 1 0 |a Rondonuwu, Ferdy S  |e author 
245 0 0 |a The Identification of Carotenoids and Testing of Carotenoid Antioxidants from Sand Lobster (Panulirus homarus) Egg Extract 
260 |b Marine Science Department Diponegoro University,   |c 2017-08-22. 
500 |a https://ejournal.undip.ac.id/index.php/ijms/article/view/15685 
520 |a Sand lobsters (Panulirus homarus) are organisms that play an important role in supporting a society's economy. Lobsters have a business appeal due to their high demand, enjoyable taste, as well as significant nutritional content. It is interesting that sand lobster eggs have a yellow orange color as an indicator of the presence of carotenoid content. Related with advancements in research and the utilization of carotenoids, this research identifies carotenoids and tests carotenoid antioxidants from sand lobster (Panulirus homarus) egg extract. In identifying carotenoids in sand lobster egg extract, HPLC (high performance liquid chromatography) (shimadzu LC-10AD, Japan) is used. In testing carotenoid antioxidants from sand lobster eggs, a DPPH method is utilized. The identification results with HPLC reveal that the sample contains carotenoids. The kinds of carotenoids identified are dinoxanthin, diadinoxanthin, zeaxanthin, lutein, astaxanthin, and violaxanthin. Among the six kinds of carotenoids identified, lutein is the type of carotenoid that has the highest concentration. These carotenoid compounds are detected in wavelengths of 400-500 nm. The test results of antioxidant power reveal that to hamper free radicals (IC50), a sample concentration of 6675.25 µg.ml-1 is needed. The ability to hinder free radicals from a sample extract is mostly conducted by lutein, zeaxanthin, and astaxantin. If seen from the analytical results, it can be surmised that sand lobster eggs contain carotenoids that can be used as a carotenoid source for humans.   Keywords : Sand Lobster, HPLC, Carotenoids, DPPH, Antioxidant 
540 |a Copyright (c) 2017 ILMU KELAUTAN: Indonesian Journal of Marine Sciences 
540 |a http://creativecommons.org/licenses/by-sa/4.0 
546 |a eng 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |2 local 
786 0 |n ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 22, No 3 (2017): Ilmu Kelautan; 155-160 
786 0 |n 2406-7598 
786 0 |n 0853-7291 
787 0 |n https://ejournal.undip.ac.id/index.php/ijms/article/view/15685/pdf 
856 4 1 |u https://ejournal.undip.ac.id/index.php/ijms/article/view/15685/pdf  |z Get Fulltext