Profil Pigmen Polar dan Non Polar Mikroalga Laut Spirulina sp. dan Potensinya sebagai Pewarna Alami (Profile of Polar and Non-Polar Pigment from Marine Microalgae Spirulina sp. and Their Potential as Natural Coloring)
Penelitian pigmen Spirulina sp. dilakukan untuk menentukan kandungan pigmen polar dan non polar dari Spirulina sp. dengan metode Spektroskopi UV-Vis. Pigmen polar diekstraksi dengan menggunakan larutan buffer fosfat (NaOH-KH2PO4) pH 7, sedangkan pigmen non polar diekstraksi dengan menggunakan aseton...
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| Format: | EJournal Article |
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Marine Science Department Diponegoro University,
2012-09-15.
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| LEADER | 03241 am a22002533u 4500 | ||
|---|---|---|---|
| 001 | IJMS_UNDIP_4696_4255 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Sedjati, Sri |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Yudiati, Ervia |e author |
| 700 | 1 | 0 | |a Suryono, Suryono |e author |
| 245 | 0 | 0 | |a Profil Pigmen Polar dan Non Polar Mikroalga Laut Spirulina sp. dan Potensinya sebagai Pewarna Alami (Profile of Polar and Non-Polar Pigment from Marine Microalgae Spirulina sp. and Their Potential as Natural Coloring) |
| 260 | |b Marine Science Department Diponegoro University, |c 2012-09-15. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/ijms/article/view/4696 | ||
| 520 | |a Penelitian pigmen Spirulina sp. dilakukan untuk menentukan kandungan pigmen polar dan non polar dari Spirulina sp. dengan metode Spektroskopi UV-Vis. Pigmen polar diekstraksi dengan menggunakan larutan buffer fosfat (NaOH-KH2PO4) pH 7, sedangkan pigmen non polar diekstraksi dengan menggunakan aseton murni. Hasil penelitian menunjukkan bahwa pigmen polar berwarna biru dan kadarnya adalah sebesar 42,272±0,05 mg/g (berat kering), pigmen ini terdiri dari fikosianin (29,159±0,29 mg/g), allofikosianin (9,363±0,20 mg/g), dan fikoeritrin (3,750±0,09 mg/g). Warna pigmen non polar adalah hijau terang dan kadarnya sebesar 4,498±0,06 mg/g (berat kering), pigmen ini terdiri dari klorofil a (3,349±0,03 mg/g) dan karotenoids (1,158±0,03 mg/g). Ekstrak pigmen polar Spirulina sp. kadarnya lebih tinggi dibanding pigmen non polarnya. Pigmen fikosianin berpotensi dikembangkan sebagai bahan aditif pewarna biru alami, karena: kadarnya paling tinggi, menghasilkan warna biru cerah dan cemerlang, bersifat nutrisi fungsional dan tidak beracun.Kata kunci: Spirulina sp., polar, non polar, pigmen, alamiResearch on pigments from Spirulina sp. powder has been done in order to determine polar and non polar pigments content by UV-Vis Spectroscopy method. Polar pigments were extracted using phosphate buffer (NaOH-KH2PO4) pH 7, while non polar pigments were extracted using pure aseton. The result showed that colour of polar pigment was dark blue and its content was 42,272±0,05 mg/g (dry weight), these pigments consist of phycocyanin (29,159±0,29 mg/g), allophycocyanin (9,363±0,20 mg/g), and phycoerythrin (3,750±0,09 mg/g). The colour of non polar pigment was light green and its content was 4,498±0,06 mg/g (dry weight), these pigments consist of chlorophyll a (3,349±0,03 mg/g) and carotenoids (1,158±0,03 mg/g). Due to the high content of pigments from Spirulina sp, showing a bright blue colour, having functional nutrition, and non toxic, the polar pigment phycocyanin have been found suitable for use as additif natural blue colorant.Key word: Spirulina sp., polar, non polar, pigment, natural | ||
| 546 | |a eng | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |2 local | |
| 786 | 0 | |n ILMU KELAUTAN: Indonesian Journal of Marine Sciences; Vol 17, No 3 (2012): Ilmu Kelautan; 176-182 | |
| 786 | 0 | |n 2406-7598 | |
| 786 | 0 | |n 0853-7291 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/ijms/article/view/4696/4255 | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/ijms/article/view/4696/4255 |z Get Fulltext |