Lignocellulosic Bioethanol Production of Napier Grass Using Trichoderma reesei and Saccharomyces cerevisiae Co-Culture Fermentation
Bioethanol from agricultural waste is an attractive way to turn waste into added value that will solve the problem of food competition and waste management. Napier grass is a highly productive and effective lignocellulosic biomass, which is an important substrate of the second-generation biofuels. I...
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| Format: | EJournal Article |
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Center of Biomass & Renewable Energy, Diponegoro University,
2022-05-05.
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| LEADER | 03013 am a22002773u 4500 | ||
|---|---|---|---|
| 001 | IJRED_UNDIP_43740_pdf | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Mueansichai, Thirawat |e author |
| 700 | 1 | 0 | |a Rangseesuriyachai, Thaneeya |e author |
| 700 | 1 | 0 | |a Thongchul, Nuttha |e author |
| 700 | 1 | 0 | |a Assabumrungrat, Suttichai |e author |
| 245 | 0 | 0 | |a Lignocellulosic Bioethanol Production of Napier Grass Using Trichoderma reesei and Saccharomyces cerevisiae Co-Culture Fermentation |
| 260 | |b Center of Biomass & Renewable Energy, Diponegoro University, |c 2022-05-05. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/ijred/article/view/43740 | ||
| 520 | |a Bioethanol from agricultural waste is an attractive way to turn waste into added value that will solve the problem of food competition and waste management. Napier grass is a highly productive and effective lignocellulosic biomass, which is an important substrate of the second-generation biofuels. In addition, several processes are required in the production of ethanol from lignocellulosic materials; thus, co-culture fermentation can shorten the production process. This experimental research utilizes Trichoderma reesei and Saccharomyces cerevisiae co-culture fermentation in the bioethanol production of Napier grass using simultaneous saccharification and fermentation technology. To improve ethanol yield, Napier grass was pretreated with 3% (w/w) sodium hydroxide. An orthogonal experimental design was employed to optimize the Napier grass content, mixed crude co-culture loading, and incubation time for maximum bioethanol production. The results showed that pretreatment increased cellulose contents from 52.85% to 82%. The optimal fermentation condition was 15 g Napier grass, 15 g mixed crude co-culture, and 7 days incubation time, which maximizes the bioethanol yield of 16.90 g/L. Furthermore, the fermentation was upscaled 20-fold, and experiments were performed with and without supplemented sugar using laboratory-scale optimal fermentation conditions. The novelty of this research lies in the use of a mixed crude co-culture of T. reesei and S. cerevisiae to produce bioethanol from Napier grass with the maximum bioethanol concentration of 25.02 and 33.24 g/L under unadded and added sugar conditions and to reduce operational step and capital costs. | ||
| 540 | |a Copyright (c) 2022 The Authors. Published by Centre of Biomass and Renewable Energy (CBIORE) | ||
| 540 | |a https://creativecommons.org/licenses/by-sa/4.0 | ||
| 546 | |a eng | ||
| 690 | |a Bioethanol; Napier grass; Trichoderma reesei; Saccharomyces cerevisiae | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |2 local | |
| 786 | 0 | |n International Journal of Renewable Energy Development; Vol 11, No 2 (2022): May 2022; 423-433 | |
| 786 | 0 | |n 2252-4940 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/ijred/article/view/43740/pdf | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/ijred/article/view/43740/pdf |z Get Fulltext |