Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia

The g.422C>T nucleotide variations in the 5' untranslated region (5'UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in...

Full description

Saved in:
Bibliographic Details
Main Authors: Anwar, S. (Author), Putra, A.C (Author), Wulandari, A.S (Author), Agung, P. P. (Author), Putra, W.P.B (Author), Said, S. (Author)
Other Authors: Center for Plant Conservation - Botanic Gardens, Indonesian Institute of Sciences (LIPI) (Contributor), Hasanuddin University (Contributor), Department of Livestock and Fisheries of Enrekang District, South Sulawesi Province (Contributor)
Format: EJournal Article
Published: Diponegoro University, 2017-08-17.
Subjects:
Online Access:Get Fulltext
Tags: Add Tag
No Tags, Be the first to tag this record!
LEADER 02894 am a22003493u 4500
001 JITAA_UNDIP_13880_pdf
042 |a dc 
100 1 0 |a Anwar, S.  |e author 
100 1 0 |a Center for Plant Conservation - Botanic Gardens, Indonesian Institute of Sciences   |q  (LIPI)   |e contributor 
100 1 0 |a Hasanuddin University  |e contributor 
100 1 0 |a Department of Livestock and Fisheries of Enrekang District, South Sulawesi Province  |e contributor 
700 1 0 |a Putra, A.C.  |e author 
700 1 0 |a Wulandari, A.S.  |e author 
700 1 0 |a Agung, P. P.  |e author 
700 1 0 |a Putra, W.P.B.  |e author 
700 1 0 |a Said, S.  |e author 
245 0 0 |a Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia 
260 |b Diponegoro University,   |c 2017-08-17. 
500 |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/13880 
520 |a The g.422C>T nucleotide variations in the 5' untranslated region (5'UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in Bali cattle populations from three different regions as the main resources of Bali cattle in Indonesia. A total of 200 head of Bali cattle have been performed genotyping on TG5 gene using polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) method and sequence analysis. Results of the study confirmed that TG5 was monomorphic in Bali cattle wherever their origin regions. Moreover, nine candidate SNPs were detected within 5'UTR of TG gene in Bali cattle compared to Genbank reference sequences, although no SNP variations among Bali cattle sample studied. The new other genetic markers within an entire TG gene suggested to be explored and verified for their polymorphisms in Bali cattle. The nine candidate SNPs were also required further verification and validation in a larger sample to be regarded as new SNPs between Bali cattle and Genbank reference sequences. 
540 |a Copyright (c) 2017 Journal of the Indonesian Tropical Animal Agriculture 
540 |a https://creativecommons.org/licenses/by-sa/4.0/ 
546 |a eng 
690 |a Bali cattle; genetic polymorphism; marbling; thyroglobulin gene; 5'-untranslated region 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Journal of the Indonesian Tropical Animal Agriculture; Vol 42, No 3 (2017): September; 175-184 
786 0 |n 2460-6278 
786 0 |n 2087-8273 
787 0 |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/13880/pdf 
856 4 1 |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/13880/pdf  |z Get Fulltext