Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circu...

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Main Authors: Maharani, D. (Author), Fathoni, A. (Author), Sumadi, S. (Author), Hartatik, T. (Author), Khusnudin, M. (Author)
Format: EJournal Article
Published: Diponegoro University, 2018-05-24.
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LEADER 02574 am a22003133u 4500
001 JITAA_UNDIP_17356_pdf
042 |a dc 
100 1 0 |a Maharani, D.  |e author 
100 1 0 |e contributor 
700 1 0 |a Fathoni, A.  |e author 
700 1 0 |a Sumadi, S.  |e author 
700 1 0 |a Hartatik, T.  |e author 
700 1 0 |a Khusnudin, M.  |e author 
245 0 0 |a Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle 
260 |b Diponegoro University,   |c 2018-05-24. 
500 |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/17356 
520 |a MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circumference (BCC), birth shoulder height (BSH), weaning body length (WBL), weaning chest circumference(WCC), weaning shoulder height (WSH) and average daily gain (ADG) were collected and used for analysis of MC4R gene. Sixty blood samples were collected for DNA isolation and PCR amplification. The single nucleotide polymorphisms (SNP) g.1133 C>G was used for genotyping by using PCR-RFLP methods. The frequenciy of G allele (0.59) was greater than C allele (0.41). The highest genotype frequencies have been detected in CG heterozygote animals (0.52) followed by GG (0.33) and CC (0.15) in homozygote animals. The results of Pearson 's Chi-square test indicated that the population was not deviate (P>0.05) from the Hardy-Weinberg equilibrium (HWE). The SNP g. 1133 C>G of MC4R gene indicated affecting high birth body length with GG genotype (P<0.05). In conclusion, the SNP g. 1133 C>G may can be a marker for birth body length of calf selection. 
540 |a Copyright (c) 2018 Journal of the Indonesian Tropical Animal Agriculture 
540 |a https://creativecommons.org/licenses/by-sa/4.0/ 
546 |a eng 
690 |a Kebumen Ongole Grade cattle; MC4R gene; body weights; body measurements 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Journal of the Indonesian Tropical Animal Agriculture; Vol 43, No 2 (2018): June; 87-93 
786 0 |n 2460-6278 
786 0 |n 2087-8273 
787 0 |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/17356/pdf 
856 4 1 |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/17356/pdf  |z Get Fulltext