Genetic polymorphism of Pit-1|HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station

The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6(g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pi...

Full description

Saved in:
Bibliographic Details
Main Authors: Hartati, H. (Author), Anwar, S. (Author), Soewandi, B.D.P (Author)
Other Authors: Indonesian Agency for Agricultural Research and Development Ministry of Agriculture, KP4S, Beef Cattle Research Station, Indonesian Research Institute for Animal Production, Research Center for Biotechnology LIPI (Contributor)
Format: EJournal Article
Published: Diponegoro University, 2018-12-11.
Subjects:
Online Access:Get Fulltext
Get Fulltext
Tags: Add Tag
No Tags, Be the first to tag this record!
LEADER 02612 am a22003013u 4500
001 JITAA_UNDIP_18162_pdf
042 |a dc 
100 1 0 |a Hartati, H.  |e author 
100 1 0 |a Indonesian Agency for Agricultural Research and Development Ministry of Agriculture, KP4S, Beef Cattle Research Station, Indonesian Research Institute for Animal Production, Research Center for Biotechnology LIPI,  |e contributor 
700 1 0 |a Anwar, S.  |e author 
700 1 0 |a Soewandi, B.D.P.  |e author 
245 0 0 |a Genetic polymorphism of Pit-1|HinfI gene in Grati-Ongole Grade cattle at Indonesian Beef Cattle Research Station 
260 |b Diponegoro University,   |c 2018-12-11. 
500 |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/18162 
520 |a The pituitary transcription factor (Pit-1) gene is one of the considered genes that responsible to growth in cattle. A specific Pit-1|HinfI gene polymorphism located within exon 6(g.1256G>A) has been shown to be associated with growth and milk traits in cattle. This study aimed to identify the Pit-1|Hinf1 gene polymorphism in Grati-Ongole Grade cattle (Grati-OG cattle). A total of 107 genomic DNA of Grati-OG cattle were used in this study. The detection of polymorphism was performed by PCR-RFLP method. Only AB and BB genotypes were succesfully identified with the frequency of 0.009 and 0.991, respectively. Whereas, the frequency of A and B alleles were 0.005 and 0.995, respectively. The observed genotype frequencies in this population do not deviated from HWE. The value of Ho, He and PIC were 0.009, 0.009 and 0.009, respectively.In conclusion, the Pit-1|HinfI gene polymorphism in Grati-OG cattle is monomorphic and hence it should not be used in further association studies. 
540 |a Copyright (c) 2018 Journal of the Indonesian Tropical Animal Agriculture 
546 |a eng 
690 |a Bos indicus; gene polymorphism; PCR-RFLP; Pit-1 gene; Ongole Grade cattle 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Journal of the Indonesian Tropical Animal Agriculture; Vol 43, No 4 (2018): December; 315-322 
786 0 |n 2460-6278 
786 0 |n 2087-8273 
787 0 |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/18162/pdf 
787 0 |n https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/18162/2266 
856 4 1 |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/18162/pdf  |z Get Fulltext 
856 4 1 |u https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/18162/2266  |z Get Fulltext