Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat
Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples w...
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Diponegoro University,
2018-12-11.
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| LEADER | 03111 am a22003373u 4500 | ||
|---|---|---|---|
| 001 | JITAA_UNDIP_19304_pdf | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Latifah, L. |e author |
| 100 | 1 | 0 | |a PMDSU-Kemenristek Dikti, Faculty of Animal S |e contributor |
| 700 | 1 | 0 | |a Maharani, D. |e author |
| 700 | 1 | 0 | |a Kustantinah, A. |e author |
| 700 | 1 | 0 | |a Hartatik, T. |e author |
| 245 | 0 | 0 | |a Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat |
| 260 | |b Diponegoro University, |c 2018-12-11. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304 | ||
| 520 | |a Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5'-TCGGGCGTCTTGTTCATCAT-3' and R: 5'-CAAGACTGGGCACTGCTTCA-3'). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat. | ||
| 540 | |a Copyright (c) 2018 Journal of the Indonesian Tropical Animal Agriculture | ||
| 546 | |a eng | ||
| 690 | |a Bligon; goat; MC4R; SNP; growth traits; association | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Journal of the Indonesian Tropical Animal Agriculture; Vol 43, No 4 (2018): December; 343-351 | |
| 786 | 0 | |n 2460-6278 | |
| 786 | 0 | |n 2087-8273 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304/pdf | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/19304/2557 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/19304/2558 | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/19304/pdf |z Get Fulltext |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/19304/2557 |z Get Fulltext |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/19304/2558 |z Get Fulltext |