Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination
This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected...
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Diponegoro University,
2020-06-09.
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| LEADER | 02755 am a22003013u 4500 | ||
|---|---|---|---|
| 001 | JITAA_UNDIP_25957_pdf | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Astuti, R.W. |e author |
| 100 | 1 | 0 | |a DRPM Kemenristekdikti |e contributor |
| 700 | 1 | 0 | |a Wijayanti, N. |e author |
| 700 | 1 | 0 | |a Haryanto, A. |e author |
| 245 | 0 | 0 | |a Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination |
| 260 | |b Diponegoro University, |c 2020-06-09. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/25957 | ||
| 520 | |a This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines. | ||
| 540 | |a Copyright (c) 2020 Journal of the Indonesian Tropical Animal Agriculture | ||
| 546 | |a eng | ||
| 690 | |a antibody response; broiler; recombinant F protein; NDV; vaccination | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Journal of the Indonesian Tropical Animal Agriculture; Vol 45, No 2 (2020): June; 78-90 | |
| 786 | 0 | |n 2460-6278 | |
| 786 | 0 | |n 2087-8273 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/25957/pdf | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/25957/4161 | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/25957/pdf |z Get Fulltext |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/downloadSuppFile/25957/4161 |z Get Fulltext |