κ-CASEIN GENE POLYMORPHISMS IN RIVERINE AND SWAMP BUFFALO IN INDONESIA
Kappa-casein (κ-casein) gene is known as a gene that plays a role in controlling milk protein andalso play a crucial role in the coagulation and curdling of milk. This study was aimed to identifypolymorphisms of the κ-Casein gene of local buffaloes in Indonesia. A total number of 40 heads ofriverine...
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| Format: | EJournal Article |
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Diponegoro University,
2014-03-15.
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| LEADER | 02585 am a22002653u 4500 | ||
|---|---|---|---|
| 001 | JITAA_UNDIP_7368_6020 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Rini, A.O. |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Sumantri, C. |e author |
| 700 | 1 | 0 | |a Damayanthi, E. |e author |
| 245 | 0 | 0 | |a κ-CASEIN GENE POLYMORPHISMS IN RIVERINE AND SWAMP BUFFALO IN INDONESIA |
| 260 | |b Diponegoro University, |c 2014-03-15. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/7368 | ||
| 520 | |a Kappa-casein (κ-casein) gene is known as a gene that plays a role in controlling milk protein andalso play a crucial role in the coagulation and curdling of milk. This study was aimed to identifypolymorphisms of the κ-Casein gene of local buffaloes in Indonesia. A total number of 40 heads ofriverine buffalo and 250 heads of swamp buffalo. This study used PCR-RFLP method, whichamplification of the κ-Casein gene resulted an amplicon with length of 157 bp, located in exon 4. Theamplified fragment were digested with EcoRV restriction enzyme, which cut the κ-Casein gene in exon4 at nucleotides of GAT|ATC, revealed the presence of one polymorphism at the base position of 23 bpthat occurs with a substitution of Ile (ATC) of the T genetic variant into Thr (ACC) of the C geneticvariant. Genotyping κ-Casein gene in riverine buffalo produced two types of allele, namely C allele (157bp) and T allele (136 and 21 bp). These two alleles resulted in three types of genotypes, namely CC, CT,and TT. Frequency of the C allele was dominant to T allele. κ-Casein gene in swamp buffalo wasmonomorphic with one allele, namely C allele. Heterozygosity value of riverine and swamp buffaloeswere low. PIC value in riverine and swamp buffalo ranged 0.000-0.288. Fixation index of κ-Casein genein riverine buffalo was low (Siborong-borong SBBC = -0,0036; Deli Serdang = -0.025), but in swampbuffalo was in fixation. This study showed that κ-Casein|EcoRV were polymorphic in riverine buffaloand monomorphic in swamp buffalo. | ||
| 546 | |a eng | ||
| 690 | |a Riverine buffalo; swamp buffalo; κ-Casein; EcoRV; PCR-RFLP; polymorphisms | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Journal of the Indonesian Tropical Animal Agriculture; Vol 39, No 1 (2014): (March); 1-9 | |
| 786 | 0 | |n 2460-6278 | |
| 786 | 0 | |n 2087-8273 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/7368/6020 | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/7368/6020 |z Get Fulltext |