EVALUATION OF LACTICIN ADDITION ON GAS PRODUCTION KINETICS AND RUMEN METHANOGENESIS in Vitro

The present study was aimed to investigate the effect of a novel bacteriocin, i.e. lacticin 3147, ongas production kinetics and methane emission under in vitro rumen environment in two consecutiveexperiments. In experiment 1, either no or 10 µM of lacticin was added to hay or hay:concentrate (1:1,w/...

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Main Authors: Jayanegara, A. (Author), Makkar, H.P.S (Author), Becker, K. (Author)
Format: EJournal Article
Published: Diponegoro University, 2014-03-15.
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LEADER 02272 am a22002653u 4500
001 JITAA_UNDIP_7375_6025
042 |a dc 
100 1 0 |a Jayanegara, A.  |e author 
100 1 0 |e contributor 
700 1 0 |a Makkar, H.P.S.  |e author 
700 1 0 |a Becker, K.  |e author 
245 0 0 |a EVALUATION OF LACTICIN ADDITION ON GAS PRODUCTION KINETICS AND RUMEN METHANOGENESIS in Vitro 
260 |b Diponegoro University,   |c 2014-03-15. 
500 |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/7375 
520 |a The present study was aimed to investigate the effect of a novel bacteriocin, i.e. lacticin 3147, ongas production kinetics and methane emission under in vitro rumen environment in two consecutiveexperiments. In experiment 1, either no or 10 µM of lacticin was added to hay or hay:concentrate (1:1,w/w) substrate. In experiment 2, the levels of lacticin additions were extended to 0, 10, 25 and 50 µM.Samples were incubated in three replicates in both experiments at 39oC for 24 h. Total gas productionand methane emission were measured during and after the incubation, respectively. Results revealedthat, in general, addition of lacticin had limited significant effects on gas production parameterscompared to control (without lacticin addition). Lacticin addition up to 50 µM did not significantlydecrease CH4 emission, although a tendency of methane reduction existed when lacticin was added at 25µM. Hay diet produced significantly less methane emission than that of hay:concentrate diet both inexperiment 1 and experiment 2 (P<0.05). It can be concluded that lacticin addition up to 50 µM wasunable to decrease CH4 emission in vitro and therefore need to be tested further at higher concentrations. 
546 |a eng 
690 |a laktisin. bakteriosin. metana. rumen. in vitro 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Journal of the Indonesian Tropical Animal Agriculture; Vol 39, No 1 (2014): (March); 30-36 
786 0 |n 2460-6278 
786 0 |n 2087-8273 
787 0 |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/7375/6025 
856 4 1 |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/7375/6025  |z Get Fulltext