DEVELOPMENT OF ENZYME-LINKAGE IMMUNOSORBENT ASSAY AGAINST TYPE B OF CLOSTRIDIUM BOTULINUM: A PRELIMINARY STUDY
Clostridium botulinum neurotoxin (BoNTs) is one of the causes of economic loss in the livestockindustry. This economic loss would be as a direct result when animals poisoned by BoNTs or indirectlywhen the livestock products are contaminated by BoNTs, which end up with the products are banned byautho...
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| Формат: | EJournal Article |
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Diponegoro University,
2011-12-15.
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| LEADER | 02283 am a22002533u 4500 | ||
|---|---|---|---|
| 001 | JITAA_UNDIP_7480_6133 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Depamede, S.N. |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Kisworo, D. |e author |
| 245 | 0 | 0 | |a DEVELOPMENT OF ENZYME-LINKAGE IMMUNOSORBENT ASSAY AGAINST TYPE B OF CLOSTRIDIUM BOTULINUM: A PRELIMINARY STUDY |
| 260 | |b Diponegoro University, |c 2011-12-15. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/7480 | ||
| 520 | |a Clostridium botulinum neurotoxin (BoNTs) is one of the causes of economic loss in the livestockindustry. This economic loss would be as a direct result when animals poisoned by BoNTs or indirectlywhen the livestock products are contaminated by BoNTs, which end up with the products are banned byauthority. Therefore a routine surveillance of BoNTs in the farm and in livestock product processingindustry is urgently needed. One of the most relatively quick and accurate methods to perform a routinedetection of the presence of BoNTs is enzyme-linkage immunosorbant assay (ELISA). In this article wedescribe the results of the development of ELISA, using polyclonal antibodies against BoNTs-Bproduced locally. Antibodies were generated from six Balb/c mice with standard immunologicalmethods. Mice were immunized three times for a period of 8 weeks with a commercial type BClostridium botulinum toxoid at a dose of 100 ng per mouse per injection. The resulting antibody waspurified by a combination of ammonium sulfate precipitation 50% (w/v) technique and a protein Acolumn method. The results of this preliminary study indicated that the developed ELISA methodcapable of detecting type B Clostridium botulinum toxin up to 1.0 ng/ml. | ||
| 546 | |a eng | ||
| 690 | |a C. botulinum toxin. ELISA. polyclonal antibody. | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Journal of the Indonesian Tropical Animal Agriculture; Vol 36, No 4 (2011): (December); 237-242 | |
| 786 | 0 | |n 2460-6278 | |
| 786 | 0 | |n 2087-8273 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/7480/6133 | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/7480/6133 |z Get Fulltext |