EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION
s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF). Each semen sample was evaluated and dividedequally...
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| Format: | EJournal Article |
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Diponegoro University,
2010-03-15.
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| LEADER | 02436 am a22002773u 4500 | ||
|---|---|---|---|
| 001 | JITAA_UNDIP_7583_6236 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Arifiantini, R.I. |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Purwantara, B. |e author |
| 700 | 1 | 0 | |a Yusuf, T.I. |e author |
| 700 | 1 | 0 | |a Sajuthi, D. |e author |
| 245 | 0 | 0 | |a EFFECT OF DIFFERENT CRYOPROTECTIVE AGENTS ON SKIM MILK AND DIMITROPOULUS EXTENDER FOR STALLION SEMEN CRYOPRESERVATION |
| 260 | |b Diponegoro University, |c 2010-03-15. | ||
| 500 | |a https://ejournal.undip.ac.id/index.php/jitaa/article/view/7583 | ||
| 520 | |a s to assess different CPAs on stallion semen cryopreservation. Skim milk (SM) and Dimitropoulos(DV) were the extenders used in this study; each was added by glycerol (Gly), combination of ethyleneglycol-glycerol (EG+Gly) or dimethilformamide (DMF). Each semen sample was evaluated and dividedequally into six tubes; semen in the three tubes was diluted 1:1 with (SM), while in the remaining tubesthe semen was diluted 1:1 by DV. After being diluted, all tubes were centrifuged at 1006xg for 10minutes. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and addedby G, EG+Gly, or DMF to reach the final sperm concentration of 200x106/ml. The extended semen wasindividually packed in 0.3 ml minitube, equilibrated at 4oC for 2 hours, frozen in liquid nitrogen vaporfor 10 minutes, and then was stored in liquid nitrogen container at -196 oC. After 24 hours, the semenwas thawed at 37 oC for 30 second. There were no significantly different (p>0.05) on the percentages ofmotile and viable sperm in SMT (21.7% and 43.4%, respectively) compared with those extended withDV T extender (26.9% and 50.8%, respectively). DMF demonstrated better results as CPA compared tothe others; and DVTDMF combination had the best protection during cryopreservation in this study. | ||
| 546 | |a eng | ||
| 690 | |a cryopreservation. stallion sperm. sugar. cryoprotective agents | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Journal of the Indonesian Tropical Animal Agriculture; Vol 35, No 1 (2010): (March); 68-74 | |
| 786 | 0 | |n 2460-6278 | |
| 786 | 0 | |n 2087-8273 | |
| 787 | 0 | |n https://ejournal.undip.ac.id/index.php/jitaa/article/view/7583/6236 | |
| 856 | 4 | 1 | |u https://ejournal.undip.ac.id/index.php/jitaa/article/view/7583/6236 |z Get Fulltext |