Effect of Green Fluorescent Protein (GFP) on the Development of Canine Intergeneric Embryo= Pengaruh Green Fluorescent Protein (GFP) Pada Perkembangan Anjing Dari Embryo Intergeneris.
The present study investigated the effect of green fluorescent protein on the development of canine intergeneric clone embryo with bovine oocyte recipient. Cumulus oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 10% (v/v) fetal bovine serum (FBS) (...
Saved in:
| Main Author: | |
|---|---|
| Format: | EJournal Article |
| Published: |
Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI,
2012-03-15.
|
| Subjects: | |
| Online Access: | Get Fulltext |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| LEADER | 01829 am a22002413u 4500 | ||
|---|---|---|---|
| 001 | JSV_UGM_356_233 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Fibrianto, Yuda Heru |e author |
| 100 | 1 | 0 | |e contributor |
| 245 | 0 | 0 | |a Effect of Green Fluorescent Protein (GFP) on the Development of Canine Intergeneric Embryo= Pengaruh Green Fluorescent Protein (GFP) Pada Perkembangan Anjing Dari Embryo Intergeneris. |
| 260 | |b Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI, |c 2012-03-15. | ||
| 500 | |a https://jurnal.ugm.ac.id/jsv/article/view/356 | ||
| 520 | |a The present study investigated the effect of green fluorescent protein on the development of canine intergeneric clone embryo with bovine oocyte recipient. Cumulus oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 10% (v/v) fetal bovine serum (FBS) (Life Technologies), 0.005 U/m1 bovine FSH (Antrin®, Denka Kanagawa, Japan) and 1 pg/m1 estradiol (Sigma-Aldrich) at 39 °C in a humidified atmosphere of 5% CO2 in air and donor cell tranfected with enhanced green fluorescent protein. In this experiment GFP have no negative effect in fusion or embryo development but the expression rate were decreased in 2 cell stage and then the expression gradually decreased with progression of embryo development. | ||
| 540 | |a Copyright (c) 2012 Jurnal Sain Veteriner | ||
| 540 | |a http://creativecommons.org/licenses/by-sa/4.0 | ||
| 546 | |a eng | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Jurnal Sain Veteriner; Vol 24, No 1 (2006): JUNI | |
| 786 | 0 | |n 2407-3733 | |
| 787 | 0 | |n https://jurnal.ugm.ac.id/jsv/article/view/356/233 | |
| 856 | 4 | 1 | |u https://jurnal.ugm.ac.id/jsv/article/view/356/233 |z Get Fulltext |