Glycerol Reduces Cross Hybridization on Nitrocellulose Membrane
Lateral flow assay (LFD) based nucleic acid lateral flow (NALF) method has been developed recently. The method met point of care testing (POCT) as simple and rapid procedures, less equipment, and can be performance by less skilled technician. NALF based on nucleic acid hybridizationis more economi...
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| Format: | EJournal Article |
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Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI,
2020-12-01.
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| LEADER | 02486 am a22003013u 4500 | ||
|---|---|---|---|
| 001 | JSV_UGM_44895_30380 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Hendarta, Narendra Yoga |e author |
| 100 | 1 | 0 | |a Prof. Dr. dr. Mulyanto and Prof. Ir. Sulaiman N. Depamede, M.Biotech, Ph.D from Hepatika Mataram and Universitas Mataram |e contributor |
| 700 | 1 | 0 | |a Aman, Abu Tholib |e author |
| 700 | 1 | 0 | |a Kusumawati, Asmarani |e author |
| 700 | 1 | 0 | |a Wibawa, Tri |e author |
| 245 | 0 | 0 | |a Glycerol Reduces Cross Hybridization on Nitrocellulose Membrane |
| 260 | |b Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI, |c 2020-12-01. | ||
| 500 | |a https://jurnal.ugm.ac.id/jsv/article/view/44895 | ||
| 520 | |a Lateral flow assay (LFD) based nucleic acid lateral flow (NALF) method has been developed recently. The method met point of care testing (POCT) as simple and rapid procedures, less equipment, and can be performance by less skilled technician. NALF based on nucleic acid hybridizationis more economical then immunochromatography assay which use antibody-antigen recognition. Cross hybridization has issued while used to differentiate organism with high GC content and high homology as high similarity genome. Some techniques has applied to give high stringency condition avoid cross hybridization reaction but need more procedure to apply. We found glycerol applied to buffer assay could reduce cross hybridization on nitrocellulose membrane. The study used 2 kinds of high stringency buffer as PBS and SSC bases and high concentration of ssDNA amplicon as sample. Without glycerol ingredient gave cross hybridization signal on test line. But used glycerol could reduce those even omitted with PBS based buffer assay. Beside those, glycerol could significantly increased hybridization signal in SSC based buffer assay (p<0.05). | ||
| 540 | |a Copyright (c) 2021 Jurnal Sain Veteriner | ||
| 540 | |a http://creativecommons.org/licenses/by-sa/4.0 | ||
| 546 | |a eng | ||
| 690 | |a Biology Molecular | ||
| 690 | |a cross-hybridization; nucleic acid lateral flow; nitrocellulose membrane; buffer | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |a Peer-reviewed Article |2 local | |
| 786 | 0 | |n Jurnal Sain Veteriner; Vol 38, No 3 (2020): Desember; 199-2015 | |
| 786 | 0 | |n 2407-3733 | |
| 787 | 0 | |n https://jurnal.ugm.ac.id/jsv/article/view/44895/30380 | |
| 856 | 4 | 1 | |u https://jurnal.ugm.ac.id/jsv/article/view/44895/30380 |z Get Fulltext |