Agrobacterium biology and its application to transgenic plant production

The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for...

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Main Author: Erh Min Lai (auth)
Other Authors: Stanton B Gelvin (auth), Hau Hsuan Hwang (auth)
Format: Book Chapter
Published: Frontiers Media SA 2015
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Online Access:Get Fullteks
DOAB: description of the publication
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020 |a 978-2-88919-574-9 
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041 0 |a English 
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100 1 |a Erh Min Lai  |4 auth 
700 1 |a Stanton B Gelvin  |4 auth 
700 1 |a Hau Hsuan Hwang  |4 auth 
245 1 0 |a Agrobacterium biology and its application to transgenic plant production 
260 |b Frontiers Media SA  |c 2015 
300 |a 1 electronic resource (165 p.) 
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520 |a The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes. 
540 |a Creative Commons  |f https://creativecommons.org/licenses/by/4.0/  |2 cc  |4 https://creativecommons.org/licenses/by/4.0/ 
546 |a English 
653 |a Agrobacterium 
653 |a crown gall 
653 |a Quorum Sensing 
653 |a plant defense 
653 |a T DNA 
653 |a Virulence 
653 |a Biofilm 
653 |a Attachment 
653 |a genetic transformation 
653 |a Membrane lipid 
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