Cell-Free Synthetic Biology

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering too...

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Bibliographic Details
Main Author: Hong, Seok Hoon (auth)
Format: Book Chapter
Published: MDPI - Multidisciplinary Digital Publishing Institute 2020
Subjects:
Online Access:Get Fullteks
DOAB: description of the publication
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100 1 |a Hong, Seok Hoon  |4 auth 
245 1 0 |a Cell-Free Synthetic Biology 
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520 |a Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas. 
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653 |a genomically engineered E. coli 
653 |a n/a 
653 |a in vitro transcription-translation (TX-TL) 
653 |a tPa 
653 |a tissue plasminogen activator 
653 |a in vitro translation 
653 |a enzymes 
653 |a DNA origami 
653 |a antimicrobials 
653 |a cell-free metabolic engineering (CFME) 
653 |a cell-free transcription-translation 
653 |a in vitro protein synthesis 
653 |a cell-free metabolic engineering 
653 |a E. coli crude extract preparation 
653 |a chaperones 
653 |a ischemic stroke 
653 |a microsomes 
653 |a sonication 
653 |a cell-free protein synthesis 
653 |a growth factors 
653 |a artificial cell 
653 |a unnatural protein 
653 |a cell-free synthetic biology 
653 |a rapid prototyping 
653 |a drug development 
653 |a unnatural amino acid 
653 |a eukaryotic lysates 
653 |a cell-free protein expression (CFPE) 
653 |a riboregulator 
653 |a synthetic biology 
653 |a mathematical model 
653 |a CFPS 
653 |a cell-free protein synthesis (CFPS) 
653 |a bioconversion platform 
653 |a colicins 
653 |a protein production 
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