Angiotensin I-converting Enzyme (ACE) Inhibitory Activity of ACE Inhibitory Peptides Produced during the Fermentation of Pigeon Pea (Cajanus cajan) Tempe

Fermentation products are common sources of angiotensin I-converting enzyme (ACE) inhibitory peptides used for hypertension treatment. This research investigated the effect of fermentation time on the ACE inhibitory activity produced during the fermentation of pigeon pea tempe and aimed to determine...

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Main Authors: Pebrianti, Suci Apsari (Author), Cahyanto, Muhammad Nur (Author), Indrati, Retno (Author)
Format: EJournal Article
Published: Indonesian Association of Food Technologists, 2020-04-16.
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LEADER 02568 am a22002893u 4500
001 ifnp_UGM_46921_27502
042 |a dc 
100 1 0 |a Pebrianti, Suci Apsari  |e author 
100 1 0 |e contributor 
700 1 0 |a Cahyanto, Muhammad Nur  |e author 
700 1 0 |a Indrati, Retno  |e author 
245 0 0 |a Angiotensin I-converting Enzyme (ACE) Inhibitory Activity of ACE Inhibitory Peptides Produced during the Fermentation of Pigeon Pea (Cajanus cajan) Tempe 
260 |b Indonesian Association of Food Technologists,   |c 2020-04-16. 
500 |a https://jurnal.ugm.ac.id/ifnp/article/view/46921 
520 |a Fermentation products are common sources of angiotensin I-converting enzyme (ACE) inhibitory peptides used for hypertension treatment. This research investigated the effect of fermentation time on the ACE inhibitory activity produced during the fermentation of pigeon pea tempe and aimed to determine the optimal fermentation time to obtain pigeon pea tempe with the highest ACE inhibitory activity. Seeds were inoculated with Raprima® (0.02% w/w) containing Rhizopus oligosporus spores and fermented for 0-96 h. Protein pattern, degree of hydrolysis (DH), soluble protein content and ACE inhibitory activity were observed during fermentation. The result from SDS-PAGE shows that protein hydrolysis occurred after 12 h fermentation, marked by the appearance and greater intensity of protein bands with low-molecular-weight (60 kDa). An increase in DH and soluble protein content were detected during the fermentation and reached a maximum of 23.99% and 3.15 mg mL -1 at 96 h fermentation, respectively. The ACE inhibitory activity increased with fermentation time and pigeon pea tempe fermented for 48 h (76.14%) has the highest ACE inhibitory activity with IC 50 values of 0.65 mg mL -1 . It could be concluded that the optimal fermentation time to obtained pigeon pea tempe with the highest ACE inhibitory activity is for 48 h of fermentation. 
540 |a Copyright (c) 2020 Suci Apsari Pebrianti 
540 |a http://creativecommons.org/licenses/by-sa/4.0 
546 |a eng 
690 |a ACE inhibitor; tempe; fermentation; pigeon pea 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Indonesian Food and Nutrition Progress; Vol 16, No 2 (2019); 47-52 
786 0 |n 2597-9388 
786 0 |n 0854-6177 
787 0 |n https://jurnal.ugm.ac.id/ifnp/article/view/46921/27502 
856 4 1 |u https://jurnal.ugm.ac.id/ifnp/article/view/46921/27502  |z Get Fullteks