Process Optimization for Recombinant Protein Expression in Insect Cells
Insect cells can be used for the efficient production of heterologous proteins. The baculovirus expression vector system (BEVS) in Spodoptera frugiperda cells and the stable transformation of Drosophila melanogaster S2 cells are widely used for this purpose. Whereas BEVS is a transient expression sy...
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| Main Authors: | , , , , |
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| Format: | Ebooks |
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IntechOpen,
2017-05-10.
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| Summary: | Insect cells can be used for the efficient production of heterologous proteins. The baculovirus expression vector system (BEVS) in Spodoptera frugiperda cells and the stable transformation of Drosophila melanogaster S2 cells are widely used for this purpose. Whereas BEVS is a transient expression system for rapid protein production, stable D. melanogaster cell lines are compatible with more complex processes modes. This chapter describes the setup of both systems, including steps for the generation of expression vectors and comprehensive optimization approaches. The genetic elements available in each system are described, as well as the use of different cloning and transfection methods and advanced process monitoring to achieve robust protein expression in larger-scale bioreactors. |
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| Item Description: | https://mts.intechopen.com/articles/show/title/process-optimization-for-recombinant-protein-expression-in-insect-cells |