Purification of Proteins: Between Meaning and Different Methods
Purification of protein attracts the scientists' attention toward science in 1926, as Somnar started purification and crystallization of urease from yeast. As years go forward, protein purification strategies updated from using dextrose passing through DEAE-cellulose and ended by affinity chrom...
Saved in:
| Main Author: | |
|---|---|
| Format: | Ebooks |
| Published: |
IntechOpen,
2019-09-02.
|
| Subjects: | |
| Online Access: | Get Online |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| LEADER | 01595 am a22001933u 4500 | ||
|---|---|---|---|
| 001 | intechopen_books_6914 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Amer, Hanaa E.A. |e author |
| 245 | 0 | 0 | |a Purification of Proteins: Between Meaning and Different Methods |
| 260 | |b IntechOpen, |c 2019-09-02. | ||
| 500 | |a https://mts.intechopen.com/articles/show/title/purification-of-proteins-between-meaning-and-different-methods | ||
| 520 | |a Purification of protein attracts the scientists' attention toward science in 1926, as Somnar started purification and crystallization of urease from yeast. As years go forward, protein purification strategies updated from using dextrose passing through DEAE-cellulose and ended by affinity chromatography. In this chapter we will describe some of the concepts and the differences between traditional and novel purification methods, in order to point out how a researcher can start the protein purification techniques. Different fundamental purification steps from plant sample will be discussed in this chapter including isolation, concentration, ion exchanger, and affinity chromatography, as well as the important additives that a researcher should add in order to gain a high purification fold. | ||
| 540 | |a https://creativecommons.org/licenses/by/3.0/ | ||
| 546 | |a en | ||
| 690 | |a Proteomics Technologies and Applications | ||
| 655 | 7 | |a Chapter, Part Of Book |2 local | |
| 786 | 0 | |n https://www.intechopen.com/books/6914 | |
| 787 | 0 | |n ISBN:978-1-78984-610-2 | |
| 856 | \ | \ | |u https://mts.intechopen.com/articles/show/title/purification-of-proteins-between-meaning-and-different-methods |z Get Online |