Passenger or Driver: Can Gene Expression Profiling Tell Us Anything about LINE-1 in Cancer?
LINE-1 retrotransposons are expressed in epithelial cancers but not normal adult tissues. Previously, we demonstrated repression of cell proliferation, migration, and invasion genes in L1-reverse transcriptase-inhibited T47D cells, while genes involved in cell projection, formation of vacuolar membr...
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2018-02-28.
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| LEADER | 02521 am a22002173u 4500 | ||
|---|---|---|---|
| 001 | intechopen_books_7204 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Ohms, Stephen |e author |
| 700 | 1 | 0 | |a Dahlstrom, Jane E. |e author |
| 700 | 1 | 0 | |a Rangasamy, Danny |e author |
| 245 | 0 | 0 | |a Passenger or Driver: Can Gene Expression Profiling Tell Us Anything about LINE-1 in Cancer? |
| 260 | |b IntechOpen, |c 2018-02-28. | ||
| 500 | |a https://mts.intechopen.com/articles/show/title/passenger-or-driver-can-gene-expression-profiling-tell-us-anything-about-line-1-in-cancer- | ||
| 520 | |a LINE-1 retrotransposons are expressed in epithelial cancers but not normal adult tissues. Previously, we demonstrated repression of cell proliferation, migration, and invasion genes in L1-reverse transcriptase-inhibited T47D cells, while genes involved in cell projection, formation of vacuolar membranes, and intercellular junctions were upregulated. Extending this, we examined microarray data from L1-silenced and Efavirenz-treated T47D cells by Weighted Gene Correlation Network Analysis and literature mining. Hub genes in the most significant module comparing L1-silenced and untreated controls included HSP90AB2p, DDX39A, PANK2, MT1M, and LIMK2. HSP90AB2p is related to HSP90, a master regulator of cancer, cancer evolvability and chemo-resistance. DDX39A is a known cancer driver gene while PANK2 and MT1M affect multiple pathways. LIMK2 and SYBL1 impact actin cytoskeletal dynamics and the cofilin pathway, cancer cell motility, and the epithelial-to-mesenchymal transition. Also affected were signal transduction, HIF1 pathways, iron/redox metabolism, stress granules and cancer stem cell-related metabolic reprogramming and the eIF4F translation initiation complex. Hub genes in other modules, including BTRC, MDM2, and FBXW7, stabilize oncoproteins like MYC, p53, and NOTCH1 or reflect CXCL12-CXCR4 signalling. Our findings support mounting evidence that L1 activity is a cause, rather than a consequence of oncogenesis, with L1 affecting the formation of cancer stem cells. | ||
| 540 | |a https://creativecommons.org/licenses/by/3.0/ | ||
| 546 | |a en | ||
| 690 | |a Gene Expression and Regulation in Mammalian Cells - Transcription Toward the Establishment of Novel Therapeutics | ||
| 655 | 7 | |a Chapter, Part Of Book |2 local | |
| 786 | 0 | |n https://www.intechopen.com/books/7204 | |
| 787 | 0 | |n ISBN:978-953-51-3867-9 | |
| 856 | \ | \ | |u https://mts.intechopen.com/articles/show/title/passenger-or-driver-can-gene-expression-profiling-tell-us-anything-about-line-1-in-cancer- |z Get Online |