Produksi Protease Alkalis Termostabil Dari Aspergillus flavus DUCC- K225 Dengan Ammonium Sulfat Sebagai Sumber Nitrogen

Protease  is a protein hydrolytic enzyme which  can be generated by a variety of microorganisms, including  mold. Aspergillus flavus K225, DUCC is indigenous mold isolated from lime soil of Madura which is  have potential as a alkaline protease  producer. This research aims was to know the effect  o...

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Main Authors: Putra, Mohammad Affan Dwica (Author), Rukmi, MG Isworo (Author), Pujiyanto, Sri (Author), Mulyani, Nies S (Author)
Format: EJournal Article
Published: Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, 2019-07-23.
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001 ioma_UNDIP__24490_15529
042 |a dc 
100 1 0 |a Putra, Mohammad Affan Dwica  |e author 
100 1 0 |e contributor 
700 1 0 |a Rukmi, MG Isworo  |e author 
700 1 0 |a Pujiyanto, Sri  |e author 
700 1 0 |a Mulyani, Nies S  |e author 
245 0 0 |a Produksi Protease Alkalis Termostabil Dari Aspergillus flavus DUCC- K225 Dengan Ammonium Sulfat Sebagai Sumber Nitrogen 
260 |b Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro,   |c 2019-07-23. 
500 |a https://ejournal.undip.ac.id/index.php/bioma/article/view/24490 
520 |a Protease  is a protein hydrolytic enzyme which  can be generated by a variety of microorganisms, including  mold. Aspergillus flavus K225, DUCC is indigenous mold isolated from lime soil of Madura which is  have potential as a alkaline protease  producer. This research aims was to know the effect  of ammonium sulfate as nitrogen source for the production of protease enzymes by Aspergillus flavus DUCC-K225. The production of alkaline  protease were conducted in  submerged culture medium with agitation. Fermentation medium used was modification Czapeks Dox Broth containing 2% casein. Incubation is carried out for 7 days.  The results showed that ammonium sulfate is a good source of nitrogen for growth and production of aklaine protease enzyme, which is demonstrated by  higher dry weight, the protease activity, the protein content and the specific activities, comparing those  on standard medium using sodium nitrate as N source.  
540 |a Copyright (c) 2019 Bioma : Berkala Ilmiah Biologi 
546 |a eng 
690 |a alkaline protease ; Aspergillus flavus ; nitrogen ; ammonium sulfat ; natrium nitrate. 
655 7 |a info:eu-repo/semantics/article  |2 local 
655 7 |a info:eu-repo/semantics/publishedVersion  |2 local 
655 7 |a Peer-reviewed Article  |2 local 
786 0 |n Bioma : Berkala Ilmiah Biologi; Vol. 21, No 1, Tahun 2019; 60-64 
786 0 |n 2598-2370 
786 0 |n 1410-8801 
787 0 |n https://ejournal.undip.ac.id/index.php/bioma/article/view/24490/15529 
856 4 1 |u https://ejournal.undip.ac.id/index.php/bioma/article/view/24490/15529  |z Get Fulltext