THE EFFECTS OF ALLOGENEIC MSC ADMINISTRATION ON ATHEROSCLEROTIC VESSEL WALL (EXPERIMENTAL STUDY OF TGF-β1, IL-1α, AND IL-6 ON ATHEROSCLEROTIC SPRAGUE DAWLEY RATS)
Background: Atherosclerosis is the leading cause of morbidity and mortality in the world. Mesenchymal stem cells (MSCs) are implicated in a variety of physiological and pathological processes. They may able to ameliorate atherosclerosis by interfering IL-1α, IL-6 and TGF-β1 expression. However, the...
Saved in:
| Main Author: | |
|---|---|
| Format: | Academic Paper |
| Published: |
2014.
|
| Subjects: | |
| Online Access: | http://eprints.undip.ac.id/48504/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background: Atherosclerosis is the leading cause of morbidity and mortality in the world. Mesenchymal stem cells (MSCs) are implicated in a variety of physiological and pathological processes. They may able to ameliorate atherosclerosis by interfering IL-1α, IL-6 and TGF-β1 expression. However, the effect of MSCs to them in atheroscherotic vessel wall has never been reported. Objective: To investigate the effect of intravenous administration of allogeneic MSCs on atherosclerotic vessel wall. Method: Sprague Dawley rats were divided into negative control group (standard diet, n=6), positive control group (atherosclerotic diet, n=6), and MSC treatment group (atherosclerotic diet and treated by single dose of 5x10 6 MSCs, n=8). MSCs were isolated by plastic adherent method from umbilical SD rats. Atherosclerotic event in abdominal aorta was stained by hematoxilin and eosin, while IL-1α, IL-6 and TGF-β1 expression were observed by immunohitochemistry. Results: Atherosclerotic plaque in the MSC treatment group was lower than in the positive control group(p=0.006). IL-1α and IL-6 expression in endothel, smooth muscle and macrophage of three groups were not different. TGF-β1 expression by macrophage inside vessel wall in MSC tretment group was lower than in positive control group (p=0.046), but there was no different of TGF-β1 expression in both endothel and smooth muscle. Conclusion: MSCs may have role in ameliorating atherosclerotic plaque in SD rats mediated in part by reducing of TGF-β1 expression in macrophage, but not by IL-1α and IL-6. Keywords: Mesenchymal stem cells, atherosclerosis, IL-1α, IL-6, TGF-β1 |
|---|---|
| Item Description: | http://eprints.undip.ac.id/48504/1/Cover.pdf http://eprints.undip.ac.id/48504/2/Chapter_1.pdf http://eprints.undip.ac.id/48504/3/Chapter_2.pdf http://eprints.undip.ac.id/48504/4/Chapter_3.pdf http://eprints.undip.ac.id/48504/5/Chapter_4.pdf http://eprints.undip.ac.id/48504/6/Chapter_5.pdf http://eprints.undip.ac.id/48504/7/Chapter_6.pdf http://eprints.undip.ac.id/48504/8/Chapter_7.pdf http://eprints.undip.ac.id/48504/9/References.pdf http://eprints.undip.ac.id/48504/10/Appendixes.pdf |