Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.)
Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbo...
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| Main Authors: | , , , , , , |
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| Format: | Academic Paper |
| Published: |
Berkala SAINSTEK,
2022-04-05T02:42:47Z.
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| Online Access: | Get Fulltext |
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| Summary: | Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies. Umbi porang (Amorphophallus muellerii BI.) merupakan salah satu jenis umbi yang memiliki kandungan glukomannan cukup tinggi sebesar 67% . Glukomannan sangat sulit dicerna oleh manusia secara langsung sehingga dibutuhkan peran probiotik. Bakteri L. casei FNCC0900 sebagai agen probiotik yang mampu memanfaatkan glukomannan sebagai sumber karbon untuk pertumbuhannya. Tujuan dari penelitian ini untuk mengetahui pola pertumbuhan dan perubahan faktor lingkungan yaitu nilai pH pada media pertumbuhan bakteri probiotik L. casei FNCC0900. Parameter dalam penelitian ini terdiri atas kepadatan sel tertinggi, waktu generasi dan perubahan nilai pH pada Media Cair Glucose Yeast Peptone, Media Air Rebusan Porang dan Media Cair Tepung Porang dengan metode drop plate yang memiliki 4 kali ulangan perhitungan. Media Cair Air Rebusan Porang memiliki periode fase log yang lebih cepat dengan kepadatan sel lebih tinggi dibandingkan Media Cair Tepung Porang, namun waktu generasi paling singkat terdapat pada Media Cair Tepung Porang dengan jumlah generasi paling tinggi. Bakteri L. casei FNCC0900 lebih mampu menurunkan pHMedia Cair Glucose Yeast Peptone dibandingkan pada media berbasis umbi porang, sehingga dalam hal ini L. casei FNCC0900 dapat dinyatakan mampu tumbuh pada media berbasis umbi porang dengan pola pertumbuhan, waktu generasi, kepadatan sel serta nilai pH yang bervariasi. |
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| Item Description: | Kodeprodi#1810401#PendidikanBiologi NIDN#0003056808 NIDN#016086012 NIDN#0003037002 NIDN#0031037401 http://repository.unej.ac.id/xmlui/handle/123456789/106119 |