Identification of antigenic proteins from salivary glands of female Anopheles maculatus by proteomic analysis
Asian Pac J Trop Biomed 2016; 6(11): 924-930
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| Main Authors: | , , , , , , |
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| Format: | Academic Paper |
| Published: |
2018-02-09T09:07:20Z.
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| Subjects: | |
| Online Access: | Get Fulltext |
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| Summary: | Asian Pac J Trop Biomed 2016; 6(11): 924-930 Objective: To identify antigenic proteins from the salivary glands of female Anopheles maculatus using a proteomic approach to find the biomarker candidate for serological tools. Methods: The identification of antigenic proteins of Anopheles maculatus salivary gland used these techniques: one-dimensional gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis), western blot, and liquid chromatography-mass spectrometry. Results: The proteins that have molecular weight (MW) 43 and 34 kDa were the antigenic protein. Computational bioinformatic analysis by Mascot Server revealed seven novel hypothetical proteins (MW: 43 kDa) and two novel hypothetical proteins (MW: 34 kDa). Further analysis (BLASTP, antigenicity, epitope mapping, and specificity analysis) showed that two novel proteins were identified as apolipoprotein D and cathepsin D in Anopheles darlingi. Conclusions: The identified proteins are potential to be developed as a biomarker of mosquito bite's exposure. |
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| Item Description: | 2221-1691 http://repository.unej.ac.id/handle/123456789/84256 |