NANOPARTIKEL MAGNETITE - EKSTRAK BIJI KARABENGUK SINTESIS (Mucuna pruriens Linn var.Utilis) INDONESIA DAN UJI POTENSINYA DALAM MENURUNKAN KATALEPSI PADA MENCIT
Pada penelitian ini dilakukan sintesis nanopartikel magnetite - ekstrak biji karabenguk (Fe-MPn) Indonesia dan uji potensinya dalam menurunkan katalepsi pada mencit. Tahapan penelitian yang dilakukan terdiri dari ekstraksi serbuk biji karabenguk, sintesis Fe-MPn, karakterisasi Fe-MPn, dan uji katale...
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| Format: | Academic Paper |
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2016-12-23.
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| Online Access: | http://repository.upi.edu/28947/ |
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| Summary: | Pada penelitian ini dilakukan sintesis nanopartikel magnetite - ekstrak biji karabenguk (Fe-MPn) Indonesia dan uji potensinya dalam menurunkan katalepsi pada mencit. Tahapan penelitian yang dilakukan terdiri dari ekstraksi serbuk biji karabenguk, sintesis Fe-MPn, karakterisasi Fe-MPn, dan uji katalepsi Fe-MPn. Ekstraksi serbuk biji karabenguk dilakukan dengan metode maserasi menggunakan pelarut etanol-air (1:1) pH 3. Sintesis Fe-MPn dilakukan dengan mereaksikan larutan ferri klorida (FeCl3) dan ekstrak biji karabenguk. Karakterisasi Fe-MPn dilakukan menggunakan instrumen Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), X-ray Diffraction (XRD) dan Thermal Gravimetric Analysis/ Differential Thermal Analysis (TG/DTA). Uji katalepsi dilakukan pada Fe-MPn dosis 5, 15, dan 25 mg/kg berat badan. Hasil penelitian menunjukkan bahwa Fe-MPn berupa agregat dengan ukuran diameter partikel rata-rata ±166,5 nm seperti ditunjukkan oleh gambar SEM. Spektrum FTIR menunjukkan pita lebar pada serapan 574,7 cm-1 mengindikasikan ikatan Fe-O dari Fe-MPn. Difraktogram X-ray menunjukkan Fe-MPn bersifat amorf. Profil TG/DTA menunjukkan penambahan partikel Fe meningkatkan kestabilan termal ekstrak biji karabenguk. Hasil uji katalepsi menunjukkan Fe-MPn pada ketiga dosis mampu menurunkan katalepsi pada mencit secara signifikan. Diantara ketiga dosis, dosis yang paling baik menurunkan katalepsi adalah dosis 15 mg/kg berat badan. Kata Kunci : Fe-MPn, katalepsi, Mucuna pruriens, nanopartikel, sintesis. The research is conducted to synthesis the nanoparticle magnetite - Indonesian velvet bean extract (Fe-MPn) and to examine its potential to decrease catalepsy symptom in mice. This study consisted of several stages i.e. extraction of velvet bean seed powder extraction, synthesis and characterization of Fe-MPn and catalepsy test of Fe-MPn. In particular, velvet bean seed powder was extracted by maceration method using ethanol-water (1:1) solvent mixture with pH 3. Fe-MPn were synthesized by reaction of ferric chloride (FeCl3) solution with velvet bean extract then were characterized by means of several methods, i.e. Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), X-ray Diffraction (XRD), and Thermal Gravimetric Analysis / Differential Thermal Analysis (TG/DTA). Catalepsy test was conducted on various doses of Fe-MPn of 5, 15, and 25 mg/kg body weight. The results showed that Fe-MPn form aggregates with the average particle diameter of size ±166,5 nm as indicated by SEM image. FTIR spectrum showed 574,7 cm-1 broad band which indicated the bond of Fe-O from Fe-MPn. X-ray diffractogram showed that Fe-MPn has amorphous structure. TG / DTA profile showed that the addition of Fe particles increase the thermal stability of velvet bean seed extract. Catalepsy test result demonstrated that the three doses of Fe-MPn can decrease catalepsy symptom in mice, significantly. The optimum dose to decrease catalepsy is 15 mg/kg body weight. Keywords: Fe-MPn, catalepsy, Mucuna pruriens, nanoparticle, synthesis. |
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| Item Description: | http://repository.upi.edu/28947/1/S_KIM_1203141_Title.pdf http://repository.upi.edu/28947/2/S_KIM_1203141_Abstract.pdf http://repository.upi.edu/28947/3/S_KIM_1203141_Table_of_content.pdf http://repository.upi.edu/28947/4/S_KIM_1203141_Chapter1.pdf http://repository.upi.edu/28947/5/S_KIM_1203141_Chapter2.pdf http://repository.upi.edu/28947/6/S_KIM_1203141_Chapter3.pdf http://repository.upi.edu/28947/7/S_KIM_1203141_Chapter4.pdf http://repository.upi.edu/28947/8/S_KIM_1203141_Chapter5.pdf http://repository.upi.edu/28947/9/S_KIM_1203141_Bibliography.pdf http://repository.upi.edu/28947/10/S_KIM_1203141_Appendix1.pdf http://repository.upi.edu/28947/11/S_KIM_1203141_Appendix2.pdf http://repository.upi.edu/28947/12/S_KIM_1203141_Appendix3.pdf |