Kinetics of the Enzymatic Hydrolysis of Sweet Cassava Starch and Bitter Cassava Flour and Gadung (Dioscorea hispida Dennst) Flour at Low Temperature
Starch is a potential substrate for this purpose, but the extra cost is needed to hydrolyze it into reducing sugar. As an alternative to the expensive and energy demanding conventional hydrolysis process, the low-temperature hydrolysis is being studied. Granular Starch Hydrolysing Enzyme (GSHE) was...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | EJournal Article |
| Published: |
Department of Chemical Engineering - Diponegoro University,
2017-08-01.
|
| Subjects: | |
| Online Access: | Get Fulltext Get Fulltext |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| LEADER | 03013 am a22003013u 4500 | ||
|---|---|---|---|
| 001 | BCREC_UNDIP_808_733 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Hargono, Hargono |e author |
| 100 | 1 | 0 | |e contributor |
| 700 | 1 | 0 | |a Jos, Bakti |e author |
| 700 | 1 | 0 | |a Kumoro, Andri Cahyo |e author |
| 245 | 0 | 0 | |a Kinetics of the Enzymatic Hydrolysis of Sweet Cassava Starch and Bitter Cassava Flour and Gadung (Dioscorea hispida Dennst) Flour at Low Temperature |
| 260 | |b Department of Chemical Engineering - Diponegoro University, |c 2017-08-01. | ||
| 500 | |a https://ejournal2.undip.ac.id/index.php/bcrec/article/view/808 | ||
| 520 | |a Starch is a potential substrate for this purpose, but the extra cost is needed to hydrolyze it into reducing sugar. As an alternative to the expensive and energy demanding conventional hydrolysis process, the low-temperature hydrolysis is being studied. Granular Starch Hydrolysing Enzyme (GSHE) was used in the process to degrade starch into reducing sugar at 30°C and pH 4. The substrates included bitter cassava flour, sweet cassava starch, and gadung flour. Starch concentrations studied were 50, 100, 150, 200, 250, 300, 350, and 400 g/L, respectively, while concentration of enzyme was 1.5 % (w/w). The optimum condition of the process was hydrolysis using 200 g/L of substrate concentration and enzyme concentration of 1.5% for 12 h. It was found that the reducing sugar was 49.3 g/L and the productivity of reducing sugar (Qrs) was 4.11 (gL-1 h-1). Lineweaver-Burk plot of Michaelis-Menten equation was used to study the inhibition kinetics. The Michaelis-Menten constants (Km) for these three substrates were determined as 141.64 g/L, 137,64 g/L and 140.84 g/L for bitter cassava flour, sweet cassava starch, and gadung flour, respectively. The value of Vm/Km, which denotes the affinity of the enzyme to the substrate, were determined and compared, and the result showed that the affinity (Vm) to the enzyme to this substrate followed the order of sweet cassava starch˃ bitter cassava flour˃ gadung flour, and all are non-competitive inhibitor, while the Ki value was 0.022 h -1. | ||
| 540 | |a Copyright (c) 2017 by Authors, Published by BCREC Group | ||
| 540 | |a http://creativecommons.org/licenses/by-sa/4.0 | ||
| 546 | |a eng | ||
| 690 | |a low temperature hydrolysis; GSHE; reducing sugar; kinetics; inhibition | ||
| 655 | 7 | |a info:eu-repo/semantics/article |2 local | |
| 655 | 7 | |a info:eu-repo/semantics/publishedVersion |2 local | |
| 655 | 7 | |2 local | |
| 786 | 0 | |n Bulletin of Chemical Reaction Engineering & Catalysis; 2017: BCREC Volume 12 Issue 2 Year 2017 (August 2017); 256-262 | |
| 786 | 0 | |n 1978-2993 | |
| 787 | 0 | |n https://ejournal2.undip.ac.id/index.php/bcrec/article/view/808/733 | |
| 787 | 0 | |n https://ejournal2.undip.ac.id/index.php/bcrec/article/downloadSuppFile/808/118 | |
| 856 | 4 | 1 | |u https://ejournal2.undip.ac.id/index.php/bcrec/article/view/808/733 |z Get Fulltext |
| 856 | 4 | 1 | |u https://ejournal2.undip.ac.id/index.php/bcrec/article/downloadSuppFile/808/118 |z Get Fulltext |