Chapter Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry

Chapter Preparing Proteoforms of Therapeutic Proteins for Top-Down Mass Spectrometry

A characteristic of many proteoforms, derived from a single gene, is their similarity regarding the composition of atoms, making their analysis very challenging. Many overexpressed recombinant proteins are strongly associated with this problem, especially recombinant therapeutic glycoproteins from l...

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Main Author: Heikaus, Laura (auth)
Other Authors: Schlüter, Hartmut (auth), Nurul Hidayah, Siti (auth), Gaikwad, Manasi (auth)
Format: Book Chapter
Published: InTechOpen 2019
Subjects:
Biochemistry
proteoforms, top-down mass spectrometry, therapeutic proteins, liquid chromatography, protein purification parameter screening, displacement chromatography
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