Guidelines for Successful Quantitative Gene Expression in Real- Time qPCR Assays

This chapter was developed to provide some important guidelines for studies with quantitative PCR (qPCR) using either dyes or probes, citing several essential components necessary for a good PCR assay. The efficiency and specificity of quantitative PCR (qPCR) depend on several parameters related to...

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Bibliographic Details
Main Authors: Rocha, Antônio José (Author), Miranda, Rafael de Souza (Author), Sousa, Antônio Juscelino Sudário (Author), Silva, André Luis Coelho da (Author)
Format: Ebooks
Published: IntechOpen, 2016-12-14.
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