Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages
A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive respon...
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| Κύριοι συγγραφείς: | , , , , |
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| Μορφή: | Academic Paper |
| Έκδοση: |
Biotechnology and Bioprocess Engineering volume,
2022-04-22T01:41:52Z.
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| Διαθέσιμο Online: | Get Fulltext |
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| Περίληψη: | A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method. |
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| Περιγραφή τεκμηρίου: | KODEPRODI2210101#Farmasi NIDN0026017801 NIDN0001026907 http://repository.unej.ac.id/xmlui/handle/123456789/106524 |