Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages
A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive respon...
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| Format: | Academic Paper |
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Biotechnology and Bioprocess Engineering volume,
2022-04-22T01:41:52Z.
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| LEADER | 01974 am a22002533u 4500 | ||
|---|---|---|---|
| 001 | repository_unej_123456789_106524 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a HIDAYAT, Mochammad Amrun |e author |
| 500 | |a KODEPRODI2210101#Farmasi | ||
| 500 | |a NIDN0026017801 | ||
| 500 | |a NIDN0001026907 | ||
| 500 | |a http://repository.unej.ac.id/xmlui/handle/123456789/106524 | ||
| 700 | 1 | 0 | |a MAHARANI, Diah Ayu |e author |
| 700 | 1 | 0 | |a PURWANTO, Djoko Agus |e author |
| 700 | 1 | 0 | |a KUSWANDI, Bambang |e author |
| 700 | 1 | 0 | |a YUWONO, Mochammad |e author |
| 245 | 0 | 0 | |a Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages |
| 260 | |b Biotechnology and Bioprocess Engineering volume, |c 2022-04-22T01:41:52Z. | ||
| 520 | |a A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method. | ||
| 546 | |a en | ||
| 690 | |a Sensitive Paper-based | ||
| 655 | 7 | |a Article |2 local | |
| 787 | 0 | |n http://repository.unej.ac.id/xmlui/handle/123456789/106524 | |
| 856 | 4 | 1 | |u http://repository.unej.ac.id/xmlui/handle/123456789/106524 |z Get Fulltext |