Purification and Characterization of Recombinant Sugarcane Sucrose Phosphate Synthase Expressed in E. coli and Insect Sf9 Cells: An Importance of the N-terminal Domain for an Allosteric Regulatory Property

Purification and Characterization of Recombinant Sugarcane Sucrose Phosphate Synthase Expressed in E. coli and Insect Sf9 Cells: An Importance of the N-terminal Domain for an Allosteric Regulatory Property

Sucrose phosphate synthase (SPS) catalyses the transfer of glycosyl group of uridine diphosphate glucose to fructose-6-phosphate to form sucrose-6-phosphate. Plant SPS plays a key role in photosynthetic carbon metabolisms, which activity is modulated by an allosteric activator glucose-6-phosphate (G...

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Main Authors: Sawitri, Widhi Dyah (Author), Narita, Hirotaka (Author), Ishizaka-Ikeda, Etsuko (Author), Sugiharto, Bambang (Author), Hase, Toshiharu (Author), Nakagawa, Atsushi (Author)
Format: Academic Paper
Published: J. Biochem. 2016;159(6):599-607, 2020-01-21T02:16:29Z.
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